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supplemented pericyte media  (ScienCell)

 
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    ScienCell supplemented pericyte media
    Supplemented Pericyte Media, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/supplemented+pericyte+media/pericyte+growth+medium/bio_rxiv__2025__01__22__634395-279-19-21
    Average 90 stars, based on 1 article reviews
    supplemented pericyte media - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Old World alphaviruses use distinct mechanisms to infect brain microvascular endothelial cells for neuroinvasion
    Article Snippet: Primary cortical human astrocytes (ScienCell, 1800) were cultured in poly-D-lysine (2 μg/cm 2 ) coated culture vessels using supplemented astrocyte media (ScienCell, 1801) according to the provider’s instructions at 37°C and 5% CO 2 . .. Primary brain vascular pericytes (ScienCell, 1200) were cultured in Poly-D-lysine (2 μg/cm 2 ) coated culture vessels using supplemented pericyte media (ScienCell, 1201) according to the provider’s instructions at 37°C and 5% CO 2 . ..



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    iPericytes are morphologically similar to HBVPs and express <t>pericyte</t> markers. A Phase contrast bright 4 × magnification images of iPSCs, HBVPs, mesoderm iPericytes and neural crest iPericytes. Scale = 200 µm. B-C Fold change gene expression measured by qPCR of pericyte genes PDGFRB, CSPG4, ACTA2 (B) and pluripotency genes OCT4 and NANOG (C) by iPSCs, neural crest iPericytes, mesoderm iPericytes and HBVPs (n = 3 per cell type). Data are normalised to HBVP cells, and comparisons were made using a one-way ANOVA: PDGFRB (F (3, 8) = 103.1, p < 0.0001), CSPG4 (F (3, 8) = 4671, p < 0.0001), ACTA2 (F (3, 8) = 9.340, p < 0.0054), OCT4 (F (3, 8) = 1686, p < 0.0001) and NANOG (F (3, 8) = 606.4, p < 0.0001). Post-hoc comparisons performed using Dunnett’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are shown as mean ± SD. D Immunocytochemistry showing expression of proteins PDGFRβ, CD13, and αSMA (green) by HBVP, mesoderm iPericytes and neural crest iPericytes. Nuclei counter-stained with DAPI (blue). Scale = 10 µm
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    iPericytes are morphologically similar to HBVPs and express <t>pericyte</t> markers. A Phase contrast bright 4 × magnification images of iPSCs, HBVPs, mesoderm iPericytes and neural crest iPericytes. Scale = 200 µm. B-C Fold change gene expression measured by qPCR of pericyte genes PDGFRB, CSPG4, ACTA2 (B) and pluripotency genes OCT4 and NANOG (C) by iPSCs, neural crest iPericytes, mesoderm iPericytes and HBVPs (n = 3 per cell type). Data are normalised to HBVP cells, and comparisons were made using a one-way ANOVA: PDGFRB (F (3, 8) = 103.1, p < 0.0001), CSPG4 (F (3, 8) = 4671, p < 0.0001), ACTA2 (F (3, 8) = 9.340, p < 0.0054), OCT4 (F (3, 8) = 1686, p < 0.0001) and NANOG (F (3, 8) = 606.4, p < 0.0001). Post-hoc comparisons performed using Dunnett’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are shown as mean ± SD. D Immunocytochemistry showing expression of proteins PDGFRβ, CD13, and αSMA (green) by HBVP, mesoderm iPericytes and neural crest iPericytes. Nuclei counter-stained with DAPI (blue). Scale = 10 µm
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    iPericytes are morphologically similar to HBVPs and express <t>pericyte</t> markers. A Phase contrast bright 4 × magnification images of iPSCs, HBVPs, mesoderm iPericytes and neural crest iPericytes. Scale = 200 µm. B-C Fold change gene expression measured by qPCR of pericyte genes PDGFRB, CSPG4, ACTA2 (B) and pluripotency genes OCT4 and NANOG (C) by iPSCs, neural crest iPericytes, mesoderm iPericytes and HBVPs (n = 3 per cell type). Data are normalised to HBVP cells, and comparisons were made using a one-way ANOVA: PDGFRB (F (3, 8) = 103.1, p < 0.0001), CSPG4 (F (3, 8) = 4671, p < 0.0001), ACTA2 (F (3, 8) = 9.340, p < 0.0054), OCT4 (F (3, 8) = 1686, p < 0.0001) and NANOG (F (3, 8) = 606.4, p < 0.0001). Post-hoc comparisons performed using Dunnett’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are shown as mean ± SD. D Immunocytochemistry showing expression of proteins PDGFRβ, CD13, and αSMA (green) by HBVP, mesoderm iPericytes and neural crest iPericytes. Nuclei counter-stained with DAPI (blue). Scale = 10 µm
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    iPericytes are morphologically similar to HBVPs and express <t>pericyte</t> markers. A Phase contrast bright 4 × magnification images of iPSCs, HBVPs, mesoderm iPericytes and neural crest iPericytes. Scale = 200 µm. B-C Fold change gene expression measured by qPCR of pericyte genes PDGFRB, CSPG4, ACTA2 (B) and pluripotency genes OCT4 and NANOG (C) by iPSCs, neural crest iPericytes, mesoderm iPericytes and HBVPs (n = 3 per cell type). Data are normalised to HBVP cells, and comparisons were made using a one-way ANOVA: PDGFRB (F (3, 8) = 103.1, p < 0.0001), CSPG4 (F (3, 8) = 4671, p < 0.0001), ACTA2 (F (3, 8) = 9.340, p < 0.0054), OCT4 (F (3, 8) = 1686, p < 0.0001) and NANOG (F (3, 8) = 606.4, p < 0.0001). Post-hoc comparisons performed using Dunnett’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are shown as mean ± SD. D Immunocytochemistry showing expression of proteins PDGFRβ, CD13, and αSMA (green) by HBVP, mesoderm iPericytes and neural crest iPericytes. Nuclei counter-stained with DAPI (blue). Scale = 10 µm
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    Image Search Results


    iPericytes are morphologically similar to HBVPs and express pericyte markers. A Phase contrast bright 4 × magnification images of iPSCs, HBVPs, mesoderm iPericytes and neural crest iPericytes. Scale = 200 µm. B-C Fold change gene expression measured by qPCR of pericyte genes PDGFRB, CSPG4, ACTA2 (B) and pluripotency genes OCT4 and NANOG (C) by iPSCs, neural crest iPericytes, mesoderm iPericytes and HBVPs (n = 3 per cell type). Data are normalised to HBVP cells, and comparisons were made using a one-way ANOVA: PDGFRB (F (3, 8) = 103.1, p < 0.0001), CSPG4 (F (3, 8) = 4671, p < 0.0001), ACTA2 (F (3, 8) = 9.340, p < 0.0054), OCT4 (F (3, 8) = 1686, p < 0.0001) and NANOG (F (3, 8) = 606.4, p < 0.0001). Post-hoc comparisons performed using Dunnett’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are shown as mean ± SD. D Immunocytochemistry showing expression of proteins PDGFRβ, CD13, and αSMA (green) by HBVP, mesoderm iPericytes and neural crest iPericytes. Nuclei counter-stained with DAPI (blue). Scale = 10 µm

    Journal: Stem Cell Research & Therapy

    Article Title: Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility

    doi: 10.1186/s13287-024-03671-x

    Figure Lengend Snippet: iPericytes are morphologically similar to HBVPs and express pericyte markers. A Phase contrast bright 4 × magnification images of iPSCs, HBVPs, mesoderm iPericytes and neural crest iPericytes. Scale = 200 µm. B-C Fold change gene expression measured by qPCR of pericyte genes PDGFRB, CSPG4, ACTA2 (B) and pluripotency genes OCT4 and NANOG (C) by iPSCs, neural crest iPericytes, mesoderm iPericytes and HBVPs (n = 3 per cell type). Data are normalised to HBVP cells, and comparisons were made using a one-way ANOVA: PDGFRB (F (3, 8) = 103.1, p < 0.0001), CSPG4 (F (3, 8) = 4671, p < 0.0001), ACTA2 (F (3, 8) = 9.340, p < 0.0054), OCT4 (F (3, 8) = 1686, p < 0.0001) and NANOG (F (3, 8) = 606.4, p < 0.0001). Post-hoc comparisons performed using Dunnett’s multiple comparisons test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data are shown as mean ± SD. D Immunocytochemistry showing expression of proteins PDGFRβ, CD13, and αSMA (green) by HBVP, mesoderm iPericytes and neural crest iPericytes. Nuclei counter-stained with DAPI (blue). Scale = 10 µm

    Article Snippet: Similar results were observed when complete pericyte media (CPM), containing specialised pericyte growth supplement (ScienCell, USA), was used compared to PM (Fig. B).

    Techniques: Gene Expression, Immunocytochemistry, Expressing, Staining

    Mesoderm iPericytes from multiple cell lines have similar mRNA expression. A Principal components analysis showing separate clustering of mesoderm iPericytes and iPSCs from n = 3 different cell lines. B Heat map showing relative expression levels in iPSCs and mesoderm iPericytes of key genes typically expressed by iPSCs, pericytes, endothelial cells (EC), microglia (MG), oligodendrocyte precursor cells (OPCs), oligodendrocytes (OL), astrocytes (AST) and neurons (NEU). Warmer colours indicate higher expression, cooler colours indicate lower expression

    Journal: Stem Cell Research & Therapy

    Article Title: Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility

    doi: 10.1186/s13287-024-03671-x

    Figure Lengend Snippet: Mesoderm iPericytes from multiple cell lines have similar mRNA expression. A Principal components analysis showing separate clustering of mesoderm iPericytes and iPSCs from n = 3 different cell lines. B Heat map showing relative expression levels in iPSCs and mesoderm iPericytes of key genes typically expressed by iPSCs, pericytes, endothelial cells (EC), microglia (MG), oligodendrocyte precursor cells (OPCs), oligodendrocytes (OL), astrocytes (AST) and neurons (NEU). Warmer colours indicate higher expression, cooler colours indicate lower expression

    Article Snippet: Similar results were observed when complete pericyte media (CPM), containing specialised pericyte growth supplement (ScienCell, USA), was used compared to PM (Fig. B).

    Techniques: Expressing

    Proliferation of iPericytes through the PDGF-BB: PDGFRβ signalling pathway. A iPericytes were incubated in basal pericyte media (PM) and treated with PDGF-BB (PM + PDGF-BB) while being exposed to 100 µM imatinib (PM + PDGF-BB + 100 µM imatinib). Proliferation was measured using an EdU uptake assay. iPericytes that are EdU-positive are indicated by magenta, while total number of iPericytes were measured by DAPI (blue). Scale bar = 50 µm. B Quantification of HBVPs, neural crest iPericytes and mesoderm iPericytes proliferating (as indicated by EdU-positive staining) as a percentage of total cells following 24 h exposure to PM, complete pericyte media with pericyte growth factors (CPM) or PM + PDGF-BB (n = 8 per condition). Data were analysed using a one-way ANOVA: HBVP (F (2, 21) = 35.52, p < 0.0001); neural crest iPericyte (F (2, 21) = 30.85, p < 0.0001); mesoderm iPericyte (F (2, 21) = 191.4, p < 0.0001). C Quantification of changes to PDGF-BB-induced proliferation with increasing concentrations of imatinib over 24 h in HBVPs, neural crest iPericytes and mesoderm iPericytes (n = 8 per condition). Data were analysed using a one-way ANOVA or Kruskal–Wallis test: HBVP (F (3, 26) = 259.2, p < 0.0001); neural crest iPericyte (H (3) = 24.41, p < 0.0001); mesoderm iPericyte (F (3, 28) = 221.5, p < 0.0001). For B , C , post-hoc comparisons were performed using Dunnett’s multiple comparisons or Dunn’s test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data shown as mean ± SD. D Heat map of key genes involved in pericyte proliferation in the PDGF-BB: PDGFRβ signalling pathway in HBVP, neural crest iPericytes and mesoderm iPericytes selected from Sweeney et al.

    Journal: Stem Cell Research & Therapy

    Article Title: Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility

    doi: 10.1186/s13287-024-03671-x

    Figure Lengend Snippet: Proliferation of iPericytes through the PDGF-BB: PDGFRβ signalling pathway. A iPericytes were incubated in basal pericyte media (PM) and treated with PDGF-BB (PM + PDGF-BB) while being exposed to 100 µM imatinib (PM + PDGF-BB + 100 µM imatinib). Proliferation was measured using an EdU uptake assay. iPericytes that are EdU-positive are indicated by magenta, while total number of iPericytes were measured by DAPI (blue). Scale bar = 50 µm. B Quantification of HBVPs, neural crest iPericytes and mesoderm iPericytes proliferating (as indicated by EdU-positive staining) as a percentage of total cells following 24 h exposure to PM, complete pericyte media with pericyte growth factors (CPM) or PM + PDGF-BB (n = 8 per condition). Data were analysed using a one-way ANOVA: HBVP (F (2, 21) = 35.52, p < 0.0001); neural crest iPericyte (F (2, 21) = 30.85, p < 0.0001); mesoderm iPericyte (F (2, 21) = 191.4, p < 0.0001). C Quantification of changes to PDGF-BB-induced proliferation with increasing concentrations of imatinib over 24 h in HBVPs, neural crest iPericytes and mesoderm iPericytes (n = 8 per condition). Data were analysed using a one-way ANOVA or Kruskal–Wallis test: HBVP (F (3, 26) = 259.2, p < 0.0001); neural crest iPericyte (H (3) = 24.41, p < 0.0001); mesoderm iPericyte (F (3, 28) = 221.5, p < 0.0001). For B , C , post-hoc comparisons were performed using Dunnett’s multiple comparisons or Dunn’s test: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. Data shown as mean ± SD. D Heat map of key genes involved in pericyte proliferation in the PDGF-BB: PDGFRβ signalling pathway in HBVP, neural crest iPericytes and mesoderm iPericytes selected from Sweeney et al.

    Article Snippet: Similar results were observed when complete pericyte media (CPM), containing specialised pericyte growth supplement (ScienCell, USA), was used compared to PM (Fig. B).

    Techniques: Incubation, Staining